Mansoureh Khojasteh Bagherzadeh, 79, had been in a coma since the strikes on Saturday assassinated Khamenei, the Tasnim news agency said.
Khamenei, whose assassination was confirmed by state media on Sunday, was a pillar of the country’s theocratic system since the Islamic revolution and saw off multiple crises over the decades, remaining defiant to the very end.
Read More: Iran confirms supreme leader Khamenei is dead after US, Israeli attacks
Israel’s military hit hundreds of targets across Iran, including strategic defence systems previously damaged in strikes last year.
It said three sites where leaders had been meeting were struck simultaneously, and several senior figures were also assassinated.